Signal amplification by glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology

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Kwon S. J., Lee K. B., Solakyıldırım K., Masuko S., Ly M., Zhang F., ...Daha Fazla

Angewandte Chemie - International Edition, cilt.51, sa.47, ss.11800-11804, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 47
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/anie.201205112
  • Dergi Adı: Angewandte Chemie - International Edition
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.11800-11804
  • Anahtar Kelimeler: carbohydrates, DNA, glycoconjugates, signal amplification, ultrasensitive detection
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır

Özet

Tiny amounts of carbohydrates (ca. 1zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.