Signal amplification by glyco-qPCR for ultrasensitive detection of carbohydrates: Applications in glycobiology
Angewandte Chemie - International Edition, cilt.51, sa.47, ss.11800-11804, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 51 Sayı: 47
- Basım Tarihi: 2012
- Doi Numarası: 10.1002/anie.201205112
- Dergi Adı: Angewandte Chemie - International Edition
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.11800-11804
- Anahtar Kelimeler: carbohydrates, DNA, glycoconjugates, signal amplification, ultrasensitive detection
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır
Özet
Tiny amounts of carbohydrates (ca. 1zmol) can be detected quantitatively by a real-time method based on the conjugation of carbohydrates with DNA markers (see picture). The proposed method (glyco-qPCR) provides uniform, ultrasensitive detection of carbohydrates, which can be applied to glycobiology, as well as carbohydrate-based drug discovery. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.