Objective: This study aimed to examine the impact of galangin, a compound recognized for its anti- inflammatory and antioxidant properties, on cell proliferation and migration using a scratch wound healing model in the L929 cell line.

Methods: In this study, we investigated the effects of different concentrations of galangin on cell proliferation and viability in the L929 cell line using the MTT method. We then examined the effects of different concentrations of galangin on wound closure in the wound line created by the scratch wound healing test. At 0, 12, 24 and 36 hours, microscopic images of the wound line were taken. Wound closure rates were calculated and a percent wound closure graph was created. At the end of our study, TGF-β levels of all groups were measured using ELISA kits.

Results: According to the viability percentages determined by the MTT method in L929 cells, 10, 25 and 50 μM concentrations of galangin significantly increased cell viability at 24, 48 and 72 hours. In the scratch wound healing model in which these three concentrations of galangin were applied as treatment, it was observed that 25 and 50 μM concentrations of galangin showed a nearly complete closure at the end of the experiment. When the measured TGF-β levels were analyzed, a significant decrease was observed in the galangin-treated groups compared to the control group.

Conclusion: In the in vitro scratch wound healing model, the observed reduction in TGF-β levels at end of the experiment in the galangin-treated groups, compared to the control group, suggests that the healing process is nearly completed, resulting in a concomitant decrease in cytokine release. These findings are consistent with the percentage closure rates assessed microscopically across the different treatment groups.