The nanoparticle synthesis by the biological method is starting a new era in nanotechnology research. Silver nanoparticles are generally synthesized by chemical and physical methods which are highly toxic and flammable. This study related to an environmentally friendly process of biosynthesis of the silver nanoparticle using Momordica charantia fruit, and their innate antioxidant and catalytic activities. Characterization of the AgNPs was done by UV-Visible Spectroscopy (UV-VIS), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). AgNO3 (5 mM) was allowed to react with an aqueous extract of M. charantia fruit. Antioxidant activity and survival rate assays were conducted by exposing Drosophila melanogaster larvae to particle concentrations at 50, 100, and 150 ppm of AgNPs. UV-VIS spectra show an absorption peak between 420 and 430 nm. The SEM images showed the size distribution of the nanoparticles and the average size was found to be 11-18 nm. Results of these analyses confirmed the formation of silver nanoparticles. Upon exposure to silver nanoparticles sized 11-18 nm, fruit fly survival rate did not exhibit a statistically significant control. However, AgNPs treatment also produced a significant increase in total antioxidant status as compared to control (p<0.05). Results indicate a protective role of AgNPs for oxidative stress. Silver nanoparticles so synthesized in this study are a simple, easy, and effective technique of nanoparticle production and synthesis of plant-mediated silver nanoparticles using M. charantia fruit extract that provides AgNPs are not toxic to fruit flies.