All living systems have the intrinsic ability to respond, to counteract and to adapt to the external and internal sources of disturbance. The aim of this study was to determine the effects of the iron chelating agent desferrioxamine (DF) and its dose combinations on heat stress in Caenorhabditis elegans (Nematodes). So, in this temperature dependent study, the protective effects of different concentrations of DF on heat stress were examined in Caenorhabditis elegans. Different concentrations of DF (100, 200, 400, 800 and 1600 μM ) were added to the food source and the worms L4 stage were transferred in these new plates. 100, 200, 400 and 800 μM DF treatment prolonged lifespan in animals grown at 35 °C. At tested concentrations, the lifespan increasing in Caenorhabditis elegans was significantly higher than lifespan in control group. On the other hand, no significant difference was observed between control group and 1600 μM DF upon lifespan in Caenorhabditis elegans. This study indicated that in the temperature and concentration dependent experiments DF has an effect of lifespan in C. elegans. © 2012 University of Bucharest.