Ensuring Research Integrity: The Critical Role Of Cell Line Authentication And Mycoplasma Testing In Biotechnology


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ALEMDAR A., TEMEL Ş. G.

European Biotechnology Congress 2024, İstanbul, Türkiye, 3 - 05 Ekim 2024, cilt.9, ss.1-4, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 9
  • Doi Numarası: 10.2478/ebtj-2025-0001
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1-4
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

Cell line authentication ensures the identity of cell lines, preventing erroneous results due to cross-contamination. Meanwhile, mycoplasma contamination can alter cell behavior and invalidate experimental outcomes. Despite these risks, many researchers had previously neglected such testing. 

Our recent survey revealed significant gaps in routine cell line authentication and mycoplasma testing among researchers. 68% had never performed a mycoplasma test, and 95% had not conducted cell line authentication before receiving free testing service developed and validated in house (patent pending). Many researchers sourced their cell lines from local or international labs, raising the risk of contamination and misidentification. 

Following the free testing, all participants found the tests valuable, with 84% willing to pay for them in the future. The tests exposed issues like misidentification and contamination, underscoring the need for regular verification. 

In conclusion, routine cell line authentication and mycoplasma testing are crucial for ensuring the accuracy and reproducibility of biotechnological research. Researchers increasingly recognize their importance, but affordable access to these services is essential for maintaining high standards in cell culture work.