Effects of some drugs on hepatic glucose 6-phosphate dehydrogenase activity in Lake Van Fish (Chalcalburnus Tarischii Pallas, 1811)

ÇİFTCİ M., TÜRKOĞLU V., Coban T. A.

JOURNAL OF HAZARDOUS MATERIALS, cilt.143, ss.415-418, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 143
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.jhazmat.2006.09.053
  • Dergi Adı: JOURNAL OF HAZARDOUS MATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.415-418
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır

Özet

Inhibitory effects of some drugs on hepatic glucose 6-phosphate dehydrogenase from Lake Van fish (chalcalburnus tarischii pallas, 1811) were investigated. For this purpose, initially liver glucose 6-phosphate dehydrogenase was purified 899-fold in a yield of 46.24% by using 2',5'-ADP Sepharose 4B affinity gel. In order to control the purification of enzyme was done SDS polyacrylamide gel electrophoresis. SDS polyacrylamide gel electrophoresis showed a single band for enzyme. A constant temperature (+4'C) was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm.