Akademik Veri Yönetim Sistemi
Turkish Society of Physiological Sciences, 46th Turkish Physiology Congress 8 – 10 October 2021 Online, İstanbul, Türkiye, 8 Ekim - 10 Kasım 2021, ss.79
AIM: White noise (WN) is a monotonous noise that comes from the external environment and includes all disturbing sounds. Noise exposure is a serious environmental stressor in living things. Although light is a powerful element in the regulation of circadian, neuroendocrine and neuro-behavior in living organisms, exposure to light in the evening delays the circadian rhythm and stresses the organism. As a result of the organism's response to stress, a large amount of free radicals are produced and changes occur in the oxidant system. Glutathione reductase (GR) catalyzes the conversion of GSH to oxidized glutathione (GSSG) in a NADPH-dependent reaction. The aim of this study is to determine the effect of environmental stressors such as white noise and light on testicular GR enzyme activity.
METHODS: Eighteen Sprag Dawley male rats obtained from ATADEM were divided into 3 groups. First group, Control group (without exposure to stress, 12/12 light-dark cycle, 24±2°C normal ambient temperature), second group WN 60' group (to white noise at 90 dB for 60 minutes/day for 14 days) and the third group WN 60'+extraday light group (60 minutes/day white noise exposure at 90 dB and 24 hours continuous light exposure every other day). At the end of the study, testicular tissues were homogenized and centrifuged. GR activities were measured spectrophotometrically.
RESULTS: GR activity; Control, WN 60' group and WN 60'+extraday light group, respectively; It was determined as 0.345 ± 0.0069 EU/mg protein, 0.246 ± 0.0049 EU/mg protein and 0.236 ± 0.0045 EU/mg protein. In the comparison between the groups, both white noise listening and light stress caused a decrease in GR activity compared to the control group (p<0.001).
CONCLUSION: The results of this study show that exposure to white noise and light stress may deteriorate the oxidative balance in the male reproductive system by reducing testicular GR activity.