Akademik Veri Yönetim Sistemi
Acarological Studies, cilt.5, sa.2, ss.69-80, 2023 (Hakemli Dergi)
3D chitin was obtained from the mite species Eustigmaeus absens Doğan (Acari: Stigmaeidae) by chemical method and silver nanoparticles were decorated on the obtained chitin. The resulting chitins were characterized by using attenuated total reflectance-Fourier Transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), energy dispersive X-ray spectrometry (EDX) and transmission electron microscopy (TEM) and their antibacterial activi-ties were evaluated. It was determined that the obtained chitin from E. absens contains carbon (C), nitrogen (N), oxygen (O) elements and trace amount of calcium (Ca) element. The characteristic amide-I and amide-II bands for α-chitin were observed by ATR-FTIR analysis. SEM images revealed that the surface of the chitin consists of macropores, micropores and broken nanofibers. TEM analysis showed that the sizes of silver nanoparticles differed between 6-20 nm. Images taken from the stereo microscope and the phase-contrast equipped light microscope showed that the chitin was obtained without disturbing the 3D structure of the organism. In addition, it was defined that silver nanoparticle decorated chitin exhibited antibacterial activities against the bacteria Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.