The effect of rutin on ovarian ischemia-reperfusion injury in a rat model.

NAYKI C., Nayki U., Cimen F., KULHAN M., YAPÇA Ö. E., Kurt N., ...More

Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, vol.34, no.9, pp.809-814, 2018 (SCI-Expanded) identifier identifier identifier


The effect of rutin on ovarian ischemia-reperfusion (I/R) injury was investigated in this experimental study. Eighteen Wistar albino female rats were divided into three groups as follows: I/R group (IRG; n=6), 50mg/kg rutin+I/R group (RG; n=6), and a healthy control group scheduled for a sham operation (SG; n=6). 2h of ischemia and following 2h of reperfusion were created in the IRG and RG by using a torsion model involving atraumatic vascular clips. Rutin, a flavonoid glycoside, was injected intraperitoneally at the dose of 50mg/kg to RG group 1h before reperfusion. Then, rats were euthanized and their ovaries were removed for biochemical and histopathological examination and also assessment of the gene expressions. IRG group had a significant increase in malondialdehyde (MDA) levels, in the expressions of tumor necrosis factor alpha (TNF-) and interleukin 1 beta (IL-1), and also in the activity of cyclooxygenase 2 (COX-2) unlike the significant decrease in total glutathione (tGSH) levels and the activity of COX-1 when compared to the SG group. However, rutin significantly decreased MDA levels, the expressions of TNF- and IL-1, and also the activity of COX-2 while it increased significantly tGSH levels and the activity of COX-1 in the RG group in comparison with the IRG group. Rutin ameliorated the I/R-induced ovarian injury in rats via its possible antioxidative and anti-inflammatory effects.