Akademik Veri Yönetim Sistemi
IV. International Academic Research Congress, Antalya, Türkiye, 30 Ekim - 03 Kasım 2018, ss.325
Introduction: With increased fuel concerns due to abating fossil fuels, rise in population and industrialization, a demand for the alternative renewable energy source has been increasing in the recent years. Various plants such as Jatropha, Pongamia, Brassica, Glycine, Helianthus, Moringa and Ricinus species have been exploited as sources for biofuel production (Atabani et al. 2012); Camelina is one such crop which is not only cost-effective but also a promising bioenergy and bio product feedstock (Moser 2010). Camelina sativa L. crantz, also known as gold of pleasure or false flax, is a potential biofuel crop belonging to the family Brassicaceae (Soriano and Narani 2012). It has been used in Europe for thousands of years as a vegetable oil and as animal feed (Zubr and Matthaus 2002). In this study, we aimed determine using Random Amplified Polymorphic-DNA (RAPD) technique the genetic differences and similarities between thirteen Camelina sativa L. varieties such as Ames 28372, CR4076/65, Ames 26665, Ames 26673, Ames 26667, CR1674/90, Ames 26676, Ames 26680, Local, Ames 26686, PI 304269, PI 250163, Vinimik 17 growing around world. Material and Methods: Plant materials were collected from different parts of World representing the species. DNA isolation was performed. The density of DNA samples was assessed in agarose gel electrophoresis, and the concentration was determined using spectrophotometer. PCR was performed with eight primers for RAPD analysis. PCR products were scored as presence (1) and absence (0) of band for each genotypes and analyzed. Data were used to calculate a Jaccard (1908) similarity index from which a UPGMA dendrogram was constructed. Results: The size of amplified fragments ranged between 300 and 2600 bp for all primers. A total of 65 bands were recorded and 58 polymorphisms were detected. A total of 89.2 % of the bands were polymorphic. Primers OPC-02 and OPY-7 gave the highest number (10) of RAPD products, primer OPW-5 and OPD-16 gave the lowest number (6) of RAPD products. The dendrogram realized from the RAPD markers grouped studied Camelina sativa species into two main clusters. The highest genetic similarity was observed between Ames 26667 and Ames 26673 while the lowest genetic similarity was observed between Ames 26667 and PI 304269, respectively. Discussion: Our results showed that there have been genetic differences between Camelina sativa varieties by RAPD technique. RAPD is a simple and efficient marker system for estimation and identification of genetic diversity among plant species. Therefore, they are valuable for detecting the genetic polymorphism of unknown, non-sequenced genomes simultaneousl.