Research Information System
International Biochemical Congress 2017 | 28th National Biochemistry, Erzurum, Turkey, 19 - 23 September 2017, vol.42, (Summary Text)
THE EFFECT OF ACUTE INFLAMMATION ON ANTIOXIDANT
SYSTEM AND VARIOUS PATHWAYS RELATED WITH
THIS SYSTEM
OBJECTIVES : Inflammation is defined as a complex biological response of body
to endogenous and exogenous s t imuli . In this study , behaves of t h e antioxidant defense system acting as a barrier to block transformation of acute
inflammation , which is beneficial for the host into chronic inflammation that
triggers a variety of pathological conditions with increased reactive oxygen
species was investigated for the first time at the molecular level.
MATERIALS -METHODS : Quantitative gene expressions of inflammatory
markers (Il-6 and Tnf-α), antioxidant enzymes , Nfkb regulating tumorigenesis
pathways , Mtdh essential for Nfkb activation , Sirt 1 playing major role in
epigenetic mechanisms and Hamp controling iron homeostasis were measured
using Real Time PCR. Metabolite levels of oxidative stress markers and total
iron content were examined . Histopathological analyzes were performed to
determine the presence of tissue damage.
RESULTS : Increase in Il-6 and Tnf -α quantitative gene expression levels
showed that model was formed . Oxidative stress formation followed by acute
inflammation was shown by reduction in GSH and increase in MDA levels .
Significant decrease in the gene expression levels of Sod, Cat, Gr, Gst and 6pgd
was observed , increase in Gpx and G6pd . Inflammatory cell infiltration ,
congestion, necrosis and intracellular edema were determined. Increase in Nfkb
and Hamp, decrease in Sirt1 and no changes in Mtdh expressions were observed.
CONCLUSIONS : It was determined that antioxidant defense system is
insufficient in oxidative stress mechanism by virtue of acute inflammation ,
however it tries to perform homeostasis.
Keywords : Inflammation , Reactive Oxygen Species , Antioxidant Defense
System, Gene Expression, Rat