4th International Congress on Plant Biology, Denizli, Türkiye, 3 - 06 Eylül 2025, ss.30, (Özet Bildiri)
Nectar is a sweet, watery, renewable secretion that mediates the interactions of plants with pollinators, herbivores, nectar and pollen thieves, and is one of the primary factors that attract pollinators to flowers. When the literature is reviewed, it is seen that different methods such as microcapillary tubes, filter paper, washing, rinsing, micropipettes and microsyringes, centrifuge and aspirator are used for nectar production. In the present study; Scrophularia xanthoglossa var. decipiens (Boiss. & Kotschy) Boiss., Scrophularia erzincanica (R.R.Mill), Astragalus pendulus (DC.), Scrophularia libanotica subsp. libanotica var. cappadocica (R.R.Mill), Psephellus pyrrhoblepharus (Boiss.) Wagenitz, Dianthus crinitus (SM.), Erysimum pulchellum (Willd.) J.Gay and Scrophularia catariifolia Boiss. & Heldr. Euphorbia denticulata Lam. by centrifugation method. and Euphorbia sp. Scrophularia rimarum Bornm., Silene montbretiana Boiss., Vaccaria pyramidata=Gypsophila vaccaria (L.) SM., Astragalus aucheri Boiss., Silene vulgaris (Moench) Garcke, Scrophularia libanotica subsp. armena R.R.Mill, Scrophularia fatmae Kandemir & Ilhan, Scrophularia libanotica subsp. libanotica var. sivasica R.R.Mill and Scrophularia canina L. species, nectar was obtained by both centrifuge and micropipette methods. In the study, the advantages and disadvantages of different nectar collection methods were observed as follows: The centrifuge method; while it is advantageous in terms of being applicable to small flowers with protected nectar and obtaining nectar in a short time, it is disadvantageous for flowers that are too large to fit into Eppendorf and Falcon tubes and for flowers of plants that secrete milky liquid when plucked. The washing method; while it is advantageous for large flowers with exposed nectar, it is disadvantageous for small flowers with protected nectar. The micropipette method; while it is advantageous for obtaining nectar without disrupting the nectar chemistry, it is disadvantageous due to its difficulty in application to small flowers, time-consuming, flowers with hairs inside the flower and those that absorb pure water.