Akademik Veri Yönetim Sistemi
Pharmacology Research and Perspectives, cilt.13, ss.1-11, 2025 (SCI-Expanded)
Lichen secondary metabolites with many remarkable biological activities are used in cancer treatments due to their low sideeffects and high anticancer potential. In particular, these metabolites constitute an interesting research area in cancer treat-ments due to their potential to induce apoptosis and suppress metastasis by inhibiting cancer-related signaling pathways.The Wnt/β- catenin signaling pathway plays a role in important biological processes such as oncogenesis, cell cycle regula-tion, cell proliferation, metastasis, differentiation, apoptosis, and drug resistance. Therefore, inhibition of this pathway isa potential target in cancer therapies. There is no detailed study explaining the potential anticancer molecular mechanismof the lichen secondary metabolite lobaric acid (LA) on breast cancer. Here, it is aimed to investigate the effect of LA onviability, apoptosis, and migration in MCF-7 cells and to elucidate the relationship between the potential anticancer effectand the Wnt/β- catenin signaling pathway. The dose- and time- dependent viability of LA-treated MCF-7 cells was evaluatedby XTT assay, and the IC 50 value was determined as 44.21 μg/mL at 48 h. LA increased the apoptotic cell population, asshown by flow cytometry analysis, qPCR, and Western blot results. LA inhibited β- catenin by inducing GSK3- β proteinexpression, thereby suppressing Wnt/β- catenin target genes. LA might be a natural active compound candidate for breastcancer treatment.