The Biochemical and Histopathological Investigation of The Effect of Thymoquinone on Methotrexate-Induced Kidney Damage in Rats
3th Eurasia Biochemical Approaches & Technologies Congress (EBAT), Antalya, Türkiye, 4 - 07 Kasım 2021, ss.166, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Antalya
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.166
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Erzincan Binali Yıldırım Üniversitesi Adresli: Evet
Özet
Methotrexate is one of the widely used antimetabolites because it inhibits cell division.1 It is a cytotoxic agent and a folic acid antagonist.2 High doses of methotrexate cause acute renal failure. Antioxidant, anti-inflammatory and chemoprotective effects of thymoquinone have been reported.3,4 The aim of this study is to biochemically and histopathologically investigate the effect of thymoquinone, which is a powerful antioxidant, in the acute kidney injury model induced by methotrexate in rats.
The study consisted of 4 groups, each including 6 rats. Groups; I. Healthy (Control), II. Methotrexate (20 mg/kg), III. Methotrexate (20 mg/kg) + Thymoquinone (20 mg/kg) and IV. Methotrexate (20 mg/kg)+ Thymoquinone (50 mg/kg) kg) form. All animals were sacrificed at the end of the 6th day.
Statistically significant differences were found between the groups in histopathological examinations (p < 0.05). While acute kidney injury was severe in the MTX group, it was mild in the MTX-20 and MTX-50 groups.
In conclusion, methotrexate causes kidney damage by oxidative damage. Thymoquinone prevents this damage with its antioxidant and other properties. Based on the findings obtained, it is revealed that thymoquinone can be used as a protective against kidney damage caused by chemotherapeutic drugs after further clinical studies.