Akademik Veri Yönetim Sistemi
7th INTERNATIONAL CONGRESS ON INNOVATIVE APPROACHES IN MEDICAL AND HEALTH SCIENCES, İstanbul, Türkiye, 4 - 05 Aralık 2025, ss.283, (Özet Bildiri)
Background: Doxorubicin (DOX) is an antineoplastic agent known for its testicular
toxicity side effect. Mechanisms of DOX-induced testicular damage include inflammation, reactive
oxygen and nitrogen species, and oxidative stress. Centella asiatica (CA) has anti-inflammatory
and antioxidant effects. The purpose of the study is to investigate the protective effects of Centella
asiatica extract aganist DOX-induced testicular toxicity in rats. Methods: The rats were randomly
assigned as four group; Healthy control (HG), Centella asiatica extract (CA), doxorubicin (DOX),
CA extract + DOX (CADX). CA extract 200 mg/kg was given orally the CA and CADX groups for
seven days. The HG and DOX groups received oral saline. One hour after, DOX 7.5 mg/kg
administered (intraperitoneally) DOX and CADX groups on days 1, 4, and 7. After that, rats were
euthanized with thiopental sodium, and tissues were removed. Oxidant/antioxidant, nitric oxide,
and cytokine levels in the tissue and hormone levels in the blood were investigated. Results: CA
extract significantly protected the DOX-induced elevation of malondialdehyde, nitric oxide, and
pro-inflammatory cytokines and reduction of superoxide dismutase and hormones. CA reduced
the severity of DOX destruction, oedema, dilatation/congestion blood vessels, connective tissue,
hemorrhage, and PMNL infiltration damage in testicular tissue from grade 3 to grade 1. Moreover,
it improved reproductive hormone dysfunction. Conclusion: CA may be beneficial in treating DOXrelated
male reproductive organ injury and dysfunction.