Akademik Veri Yönetim Sistemi
ULUSLARARASI 9. İLAÇ KİMYASI KONGRESİ (9th Drug Chemistry Conference), Antalya, Türkiye, 8 - 11 Nisan 2021, ss.1-2
Plants belonging to the ethnobotanical class, also known as medicinal and aromatic plants, have been
used for various purposes from past to present. Due to the possible side effects of synthetic drugs used
in modern medicine, there is an increasing interest in natural and herbal therapy in developing
countries. In recent years, the application of the extracts prepared from medicinal plants or secondary
metabolites isolated from such plants has increased in modern preclinical studies, especially in cancer
treatment. Hypericaceae family contains more than 400 species in various parts of the world including
Turkey. Hypericaceae species, especially Hypericum linarioides Bosse are used as folk medicinal plant
in our country and in many parts of the world. Although there are some biological activity studies on
different types of the reinforcement, there are no studies on the phytochemical content of Hypericum
linarioides Bosse and anticancer studies. In this study, phenolic composition, antioxidant activity,
cytotoxicity and apoptotic effects of various extracts (hexane, methanol and water) from the aerial
parts of Hypericum linarioides Bosse were investigated. Total phenolic (TPC) and flavonoid (TFC)
contents of the extracts were determined by Folin ciocalteu and aluminium chloride colorimetric
assays, respectively. Antioxidant activity was evaluated by DPPH radical scavenging and ferric ion
reducing antioxidant power (FRAP) assays. Cytotoxicty of the extracts was investigated over human
colorectal adenocarcinoma (HT-29) and human breast adenocarcinoma (MCF-7) cells by XTT assay.
Apoptotic properties was determined by flow cytometry analysis, respectively. TPC, TFC, DPPH, FRAP,
and XTT assay results demonstrated that the methanolic extract has the highest antioxidant and
antiproliferative activity with the highest phenolic and flavonoid content and the lowest IC50 values,
followed by water and hexane extracts, respectively. Flow cytometric analysis with methanol extract
showed that this extract directed HT-29 cells to apoptosis. The results of the present study provide a
significant basis for the utilization of Hypericum linarioides Bosse as an alternative to
chemotherapeutic drugs used in the treatment of breast and colon cancer with lower side effects as
well as being a source for other preclinical studies to be performed with this species.
Acknowledgement: This work was supported by Erzincan Binali Yıldırım University Scientific Research Project
(Project Number: FBA-2020-736).