Effects of nicotine and vitamin E on carbonic anhydrase activity in some rat tissues in vivo and in vitro


Çiftçi M., Bülbül M., GÜL M., Gümüştekin K., Dane Ş., Süleyman H.

Journal of Enzyme Inhibition and Medicinal Chemistry, vol.20, no.1, pp.103-109, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1080/14756360400002098
  • Journal Name: Journal of Enzyme Inhibition and Medicinal Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.103-109
  • Keywords: Carbonic anhydrase, Hippophea rhamnoides, Inhibition, Nicotine
  • Erzincan Binali Yildirim University Affiliated: No

Abstract

Effects of nicotine, nicotine + vitamin E and nicotine + Hippophea rhamnoides L. extract (HRe-1) on muscle, heart, lungs, testicle, kidney, stomach, brain and liver carbonic anhydrase (CA; EC 4.2.1.1.) enzyme activities were investigated in vivo. Groups of rats were given nicotine (0.5 mg/kg/day, i.p.), nicotine + vitamin E (75 mg/kg/day, i.g.), nicotine + HRe-1 (250 mg/kg/day, i.g.) and a control group vehicle only. The results showed that nicotine inhibited the heart, lung, stomach and liver CA enzyme activities by ∼80% (p < 0.001), ∼94% (p < 0.001), ∼47% (p < 0.001) and ∼81% (p < 0.001) respectively, and activated muscle and kidney, but had no effects on the testicle and brain CA activities. Nicotine + vitamin E inhibited the heart and liver CA enzyme activities by ∼50% (p < 0.001), and ∼50% (p < 0.001), respectively, and nicotine + vitamin E activated the muscle CA activity. However, nicotine + vitamin E had no effect on lung, testicle, kidney, stomach and brain CA activities. Nicotine + HRe-1 inhibited the heart and stomach CA enzyme activities by ∼51% (p < 0.001), and ∼32% (p < 0.002), respectively, and activated the muscle and brain CA activities, but had no effects on the lung, testicle, kidney, and liver CA activities. In vitro CA inhibition results for similar experiments correlated well with the in vivo experimental results in lungs, testicles, kidney, stomach, brain and liver tissues. © 2005 Taylor & Francis Ltd.