Antigenic specificity was investigated in dogs experimentally infected with Taenia hydatigena using SDS-PAGE and Western blotting. Cysticercus tenuicollis cysts from sheep were given to the puppies in the experimental group. Experimental infection was achieved in the animals of the experimental group at 11-13 weeks, and infected animals were necropsied and mature Taenia hydatigena worms were collected. Antigen was prepared from the fluids of C. tenuicollis. Sera from both experimental and control groups were tested by Western blotting. According to the results of this study performed with fluid antigen from C tenuicollis, the bands of 38 and 42.5 kDa are concluded to be the specific proteins for diagnosis of T. hydatigena in dogs.