Akademik Veri Yönetim Sistemi
8. International Mediterranean Scientific Research Congress, Antalya, Türkiye, 13 - 15 Ağustos 2025, ss.30-31, (Özet Bildiri)
Medicinal plants have historically constituted integral components of traditional medical systems and are now widely recognized as promising sources for the discovery of natural therapeutic agents with favorable safety profiles. Secondary metabolites such as terpenoids, phenolic acids, flavonoids, coumarins, and alkaloids are well-documented for their antioxidant and anticancer activities in the literature. In this study, the phytochemical profile and biological effects of methanolic extracts derived from the aerial parts of Hedysarum yilmazunalii a locally endemic species from Erzincan traditionally used in Chinese herbal medicine were investigated. Quantitative phytochemical analysis showed total phenolic and flavonoid contents of 108.67±0.28 mg GAE/g and 25.36±0.36 mg QE/g, respectively. Antioxidant activity was evaluated using the DPPH· radical scavenging assay and the FRAP method, with an IC50 value of 19.77±1.70 μg/mL and a FRAP value of 178.71±0.47 μg TE/g. Comparative analysis with standard antioxidants (Trolox, BHA, BHT, and ascorbic acid) further supported the interpretation of these results. Antiproliferative activity was assessed in human lung cancer cell lines (A549, Calu‑1, H1650) and a normal bronchial epithelial line (Beas‑2B). The extract exhibited potent antiproliferative effects (GI50 < 10 μg/mL), with the strongest growth inhibition observed in H1650 cells (TGI=9.57 μg/mL). LC50 values were found to be higher than 500 μg/mL, indicating a low level of general cytotoxicity. In addition, the Tumor Selectivity Index (TSI) was calculated as >2, supporting the selective cytotoxic potential of the extract. LC-MS/MS profiling was used to identify and quantify 56 phenolic compounds, with quinic acid and coumarin identified as the major constituents. According to previous studies, quinic acid has been reported to exhibit antioxidant, antidiabetic, anticancer, and anti-aging activities. Notably, its anticancer mechanism has been suggested to involve the inhibition of AP‑1, PKC, and MAPK signaling pathways, as well as the suppression of MMP‑9 expression. Overall, the findings indicate that Hedysarum yilmazunalii is an endemic species characterized by high levels of phenolic and flavonoid compounds, with selective anticancer activity and low toxicity supporting its potential as a phytochemical resource for future drug development.