Research Information System
Iranian Journal Of Pharmaceutical Research, vol.20, no.4, pp.422-434, 2021 (SCI-Expanded)
In the present study, the phenolic content by using LC MS/MS method, anticholinergic,
antioxidant (metal reduction, radical and removal of lipid peroxidation), and antibacterial
activities of Erica manipuliflora Salisb. (EMS) extract were determined. The amount of vanillic
acid, fumaric acid, catechin hydrate, quercetin, and phloridzin dihydrate were found higher
than other compounds. The ethanol extract of the EMS showed an inhibition effect on the
Acetylcholinesterase (AChE) enzyme with IC50 value of 0.124 ± 0.008 mg mL-1. Also, this extract
of the EMS indicated radical (DPPH and ABTS) scavenging activity (about 20%) and the reducing
capacity for Cu(II) and Fe(III) close to trolox, and inhibited the oxidation of linoleic acid with
40.5% at 20 μg mL-1. Antibacterial activity of the extracts was investigated against Staphylococcus
aureus, Escherichia coli, and Salmonella Typhimurium using agar disc diffusion and minimum
inhibitory concentration (MIC) methods. The EMS extract was found to be effective when used
at least 312 mg mL-1 concentration on the pathogenic microorganisms. Consequently, it has an
important non-synthetic natural content that can be used in the treatment of many diseases due
to its many bioactivities such as anticholinergic, antioxidant (radical removal, lipid peroxidation
prevention, etc.) and antibacterial.