Hypericum linarioides Bosse-(Kan Otu)'nun İnsan Meme Kanseri (MCF-7) Hücreleri Üzerine Antioksidan ve Sitotoksik Etkileri


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Altay A., Kağan Yeniçeri E. K.

EURASIANBIOCHEM , Ankara, Turkey, 24 - 26 November 2021, pp.712-720

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.712-720
  • Erzincan Binali Yildirim University Affiliated: Yes

Abstract

In this study, total phenolic and flavonoid contents, antioxidant activity and cytotoxic effects of hexane

and methanol extracts obtained from the aerial parts of Hypericum linarioides Bosse (H. linarioides)

were investigated. Total phenolic and total flavonoid contents of the extracts were determined by Folin

ciocalteu and aluminum chloride colorimetric analysis, respectively. Antioxidant activity was evaluated

by DPPH radical scavenging and iron ion reducing antioxidant power (FRAP) assays. The cytotoxicity

of the extracts was investigated by XTT assay on human breast adenocarcinoma (MCF-7) cell line. Folin

ciocalteu and aluminum chloride studies showed that methanolic extract was richer in phenolic and

flavonoid contents. In parallel to phenolic contents, methanolic extract exhibited much higher DPPH

radical scavenging and iron ion reduction capacity. XTT analysis results revealed that the methanolic

extract exhibited much stronger cytotoxicity against the tested MCF-7 cancerous cells with its much

lower IC50 values. In conclusion, all these results suggest that H. linarioides may be an important basis

for using as an alternative to chemotherapeutic drugs used in breast cancer treatment with less side

effects and also can be a source for other preclinical studies to be conducted with this species.

Keywords: Hypericum linarioides Bosse, Breast cancer, Antioxidant activity, Cytotoxicity