High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis
Analytical Biochemistry, cilt.401, sa.2, ss.236-241, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 401 Sayı: 2
- Basım Tarihi: 2010
- Doi Numarası: 10.1016/j.ab.2010.03.004
- Dergi Adı: Analytical Biochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.236-241
- Anahtar Kelimeler: ESI-FTMS, Heparin oligosaccharides, Preparative PAGE
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır
Özet
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies. © 2010 Elsevier Inc.