5th INTERNATIONAL ISTANBUL SCIENTIFIC RESEARCH CONGRESS, İstanbul, Türkiye, 14 - 15 Ağustos 2021, ss.34
The rate of cross pollination in pumpkins is very high. Due to cross
pollination, different lines may appear from the original seed. The genetic
diversity of 14 local winter squash genotypes collected from Erzincan province
was investigated. SSR (Simple Sequence Repeat) markers were used to determine
the genetic diversity of these genotypes. The 7 SSR markers used produced a
total of 15 polymorphic bands, the number of alleles per marker ranged from 2
to 5, and the mean number of alleles was 3.28. Gene diversity per marker was
found to be an average of 0.155. The mean of Nei gene diversity (h) was 0.477.
The polymorphic information content (PIC) ranged from 0.00 (GMT-M30) to 0.202
(GMT-P25) and the average PIC value per token was 0.152. Cluster analysis using
Nei's genetic distance determined that 14 genotypes were divided into 3 main
groups. In this study, it was aimed to determine the SSR markers and the degree
of genetic relatedness at the molecular level of some local winter squash
genotypes grown in Erzincan province. The results of this study show that SSR
analysis can be used successfully for the estimation of genetic diversity among
winter squash genotypes and that this genetic material, whose diversity has
been determined, can potentially be included in future studies.
studies.