Akademik Veri Yönetim Sistemi
3rd International Mardin Scientifıic Research and Innovation Congress, Mardin, Türkiye, 28 - 29 Haziran 2025, ss.582-583, (Özet Bildiri)
Medicinal plants have long been recognized as essential components of traditional medical systems and continue to receive scientific attention as promising sources of natural, low-toxicity therapeutic agents. Secondary metabolites identified in these plants such as terpenoids, phenolic acids, flavonoids, coumarins, and alkaloids are widely reported to possess antioxidant and anticancer properties, attracting growing interest in pharmacological research. These bioactive constituents have been reported to exhibit pharmacological activity and reduced treatmentassociated toxicity, particularly in cancer therapy. In this study, the phenolic and flavonoid contents, along with the antioxidant and anticancer potential, of the methanolic extract obtained from the aerial parts of Chenopodium foliosum (Moench) Asch. a species naturally found in Turkish flora and used in traditional medicine were investigated. The total phenolic and flavonoid contents were 50.33 ± 0.28 mg GAE/g and 14.52 ± 0.55 mg QE/g, respectively. Antioxidant activity was determined via the FRAP assay (45.21 ± 0.20 μg TE/g), while the DPPH assay yielded an IC₅₀ of 147.96 ± 1.57 μg/mL. Anticancer activity was determined in A549, Calu1, and H1650 human lung cancer cell lines and the Beas2B normal bronchial epithelial line. GI₅₀ values <10 μg/mL indicated strong antiproliferative activity. TGI values were 122.07 μg/mL for A549, 40.71 μg/mL for Calu1, and 19.37 μg/mL for H1650. Calu1 and H1650 showed greater sensitivity than cells treated with 5-fluorouracil. LC₅₀ values >500 μg/mL suggested low cytotoxicity. In Beas2B cells, the extract's TGI was 292.60 μg/mL versus 112.19 μg/mL for 5-FU. A TSI >2 confirmed selective cytotoxicity. Except in A549, the extract induced higher membrane toxicity than 5-FU. These findings are consistent with previously reported pharmacological activities of other species within the Chenopodium genus, supporting that C. foliosum represents a potential source of phenolic and flavonoid compounds with selective anticancer activity and low offtarget cytotoxicity. This study highlights its potential as a promising endemic phytochemical reservoir for the development of novel natural anticancer agents.