Effect of Adenosine Triphosphate on Methanol Induced Oxidative Liver Injury in Rats


Koç Y., Mertoğlu C., Süleyman H., Çankaya M., Çoban T. A.

2 nd Eurasia Biochemical Approaches & Technologies (EBAT) 2019 , Antalya, Turkey, 26 - 29 October 2019, pp.169

  • Publication Type: Conference Paper / Summary Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.169
  • Erzincan Binali Yildirim University Affiliated: Yes

Abstract

Methanol (methyl alcohol) is a colorless, volatile and a type of alcohol whose metabolites are toxic. In our country, it has been found that methyl alcohol intoxication has been observed to a certain level over the years. Methyl alcohol has serious clinical consequences, from blindness to death. The primary toxic factor in methyl alcohol intoxication is metabolic acidosis. Many studies have investigated the effect of methyl alcohol on hepatotoxicity due to oxidative damage. Although adenosine triphosphate (ATP) has been shown to be effective in studies on ischemic tissue to reduce oxidative damage, there is no study on its effect on oxidative damage in methyl alcohol-induced hepatotoxicity. In this context, we aimed to investigate the protective effects of ATP on alcoholic acute liver injury explained by oxidative stress in rats. A total of 24 rats were randomly divided into 3 groups. Group 1 was selected as the control group and no procedure was performed. After oral administration of methotrexate 0.3 mg / kg by gavage to groups 2 and 3 for 7 days, 20% methanol was given in the same route at 3 g / kg. Blood was taken from the tail veins of the animals for the measurement of serum ALT and AST 8 hours after ATP injection. Subsequently, the animals were sacrificed by high dose (50 mg / kg) thiopental anesthesia and histopathological examinations were performed on the excised liver tissues. ALT, AST values and AST / ALT ratio were found to be higher in the methanol-treated group than the other two groups and higher in the methanol + ATP group than the control group (p <0.001). When the tissues were evaluated histopathologically; normal polygonal cells with prominent round nuclei and eosinophilic cytoplasm were observed in the liver control tissue. Kupffer cells were distributed between hepatocyte cell cords and the vessels were in normal structure. The most significant change in the MTO group was hepatocyte degeneration and hepatic cord separation. Hepatocytes were swollen and showed severe hypertrophy, Kupffer cell number was relatively increased and hepatocyte cytoplasms contained vacuoles. Polymorphonuclear cell infiltration was also observed around the central vein. Hepatic cords were normal in MAP group and degeneration in hepatocytes was low. Kupffer cells were relatively reduced in number and a small amount of polymorphonuclear cells were observed in the central veins. As a result, methanol hepatotoxicity can be reduced with ATP treatment. This investigation suported by Erzincan Binali Yıldırım University Scientific Research Fund (TYL-2019-618)