Research Information System
2 nd Eurasia Biochemical Approaches & Technologies (EBAT) 2019 , Antalya, Turkey, 26 - 29 October 2019, pp.169
Methanol (methyl alcohol) is a colorless, volatile and a type of alcohol whose metabolites are toxic.
In our country, it has been found that methyl alcohol intoxication has been observed to a certain level over
the years. Methyl alcohol has serious clinical consequences, from blindness to death. The primary toxic
factor in methyl alcohol intoxication is metabolic acidosis. Many studies have investigated the effect of
methyl alcohol on hepatotoxicity due to oxidative damage. Although adenosine triphosphate (ATP) has
been shown to be effective in studies on ischemic tissue to reduce oxidative damage, there is no study on
its effect on oxidative damage in methyl alcohol-induced hepatotoxicity. In this context, we aimed to
investigate the protective effects of ATP on alcoholic acute liver injury explained by oxidative stress in
rats.
A total of 24 rats were randomly divided into 3 groups. Group 1 was selected as the control group
and no procedure was performed. After oral administration of methotrexate 0.3 mg / kg by gavage to
groups 2 and 3 for 7 days, 20% methanol was given in the same route at 3 g / kg. Blood was taken from the
tail veins of the animals for the measurement of serum ALT and AST 8 hours after ATP injection.
Subsequently, the animals were sacrificed by high dose (50 mg / kg) thiopental anesthesia and
histopathological examinations were performed on the excised liver tissues.
ALT, AST values and AST / ALT ratio were found to be higher in the methanol-treated group than
the other two groups and higher in the methanol + ATP group than the control group (p <0.001).
When the tissues were evaluated histopathologically; normal polygonal cells with prominent round nuclei
and eosinophilic cytoplasm were observed in the liver control tissue. Kupffer cells were distributed
between hepatocyte cell cords and the vessels were in normal structure. The most significant change in the
MTO group was hepatocyte degeneration and hepatic cord separation. Hepatocytes were swollen and
showed severe hypertrophy, Kupffer cell number was relatively increased and hepatocyte cytoplasms
contained vacuoles. Polymorphonuclear cell infiltration was also observed around the central vein.
Hepatic cords were normal in MAP group and degeneration in hepatocytes was low. Kupffer cells were
relatively reduced in number and a small amount of polymorphonuclear cells were observed in the central
veins. As a result, methanol hepatotoxicity can be reduced with ATP treatment.
This investigation suported by Erzincan Binali Yıldırım University Scientific Research Fund
(TYL-2019-618)