Effect of Mirtazapine on Gastric Oxidative Stress and DNA Injury Created With Methotrexate in Rats

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Demiryilmaz I., UZKESER H., Cetin N., HACIMÜFTÜOĞLU A., BAKAN E., Altuner D.

ASIAN JOURNAL OF CHEMISTRY, vol.25, no.4, pp.2047-2050, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.14233/ajchem.2013.13296
  • Journal Name: ASIAN JOURNAL OF CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2047-2050
  • Erzincan Binali Yildirim University Affiliated: No

Abstract

In this study, effect of mirtazapine on gastric oxidative stress and DNA injury created with methotrexate was investigated. Experimental results showed that GSH (nmol/g protein), MDA (mu mol/g protein) and MPO (mu/g protein) in the gastric tissue of the control group rats receiving methotexate are 4.97 +/- 0.37, 2.78 +/- 0.30 and 3.12 +/- 0.18, respectively. GSH, MDA and MPO measurements in the gastric tissue of rats receiving mirtazapine + methotrexate were detected to be 9.23 +/- 0.51(p < 0.0001), 1.80 +/- 0.31(p < 0.0001) and 1.63 +/- 0.25 (p < 0.0001), respectively. GSH, MDA and MPO values in the intact rat group were found 8 +/- 0.38 (p < 0,0001), 1.63 +/- 0.28 (p < 0.0001) and 1.37 +/- 0.21 (p < 0.0001), respectively. In addition, while 8-ohdG/dG quantity that DNA injury product in the control group administered methotrexate was 2.4 +/- 0.11 pmol/L, this quantity was 1.3 +/- 0.14 pmol/L (p < 0.001), 1.1 +/- 0.10 pmol/L (p < 0.001) in mirtazapine and intact group, respectively. As a result, it was seen that mirtazapine prevents increase of oxidative stress and DNA injury created with methotreaxete in the gastric tissue of rat.