Research Information System
3rd International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2021) December 15-17, 2021 www.EurasianSciEnTech.org, Ankara, Turkey, 15 - 17 December 2021, pp.123
Alzheimer's is a
very common dementia disease recently and there is no definite cure. In
Alzheimer's disease, which causes a decrease in neurotransmitters in the brain,
the decrease in acetylcholine neurotransmitter is seen the most. To treat or
prevent the disease, the degradation of acetylcholine should be reduced, and
this is possible with the inhibition of acetylcholinesterase (AChE), which breaks down acetylcholine.1
To investigate the in
vitro effect of naphthalenylmethylen hydrazine derivative compounds on AChE
enzyme, first, pure enzyme was supplied ready. Enzyme activity was determined
according to the method performed by Ellman et al.2 Acetylthiocholine
iodate (AChI) was used as substrate. The yellow colored 5-thio-2-nitrobenzoate
anion, which is formed because of hydrolysis of the substrates and then the
reaction of DTNB with thiocholine, gives maximum absorbance at 412 nm
wavelength.3
The inhibition effects
of novel naphthalenylmethylen hydrazine derivative compounds (1a-1h) used in
the study on the AChE enzyme were determined and the IC50 value at
the nano molar level was calculated. It was observed that these compounds
inhibited the enzyme significantly. In our study, IC50 values were
determined to be in the range of 21.3-669.7 nM. Compared to the control
compound Tacrine (IC50: 28.6 nM), 1c compound showed the best
inhibitory effect.