NEW ZEALAND JOURNAL OF BOTANY, cilt.64, sa.1, ss.70032, 2026 (SCI-Expanded, Scopus)
Satureja hortensis, widely used in traditional medicine and culinary applications, is a valuable aromatic plant rich in essential oils(Eos) and bioactive compounds. This study comprehensively evaluated the phytochemical composition and biological propertiesof S. hortensis grown in Erzincan, Turkey. The EO and four solvent-based extracts (ethyl acetate, butanol, water extract (WE), andwater fraction) were analyzed. GC–MS/MS identified 34 volatile compounds (98.76%), with thymol (40.83%), carvacrol (19.77%),γ-terpinene (16.71%), and p-cymene (12.06%) as major constituents. LC-ESI-MS/MS revealed phenolic compounds, with hesperi-din, rutin, and caffeic acid as major constituents in ethyl acetate and butanol fractions. Antioxidant activity was determined byDPPH, ferric reducing antioxidant power (FRAP), TPC, and TFC assays. The ethyl acetate showed the strongest DPPH scav-enging (17.25 ± 2.34 μg/mL), highest TPC (79.24 ± 0.59 mg gallic acid equivalent /g), and moderate FRAP activity (96.86 ±1.06 mg TE/g), while the WE had the highest flavonoid content (48.21 ± 1.56 mg QE/g). The EO exhibited potent antimicrobialactivity (MIC 31.25–250 μg/mL; maximum inhibition zones 50 mm) against A. niger, C. albicans, and A. alternata. HPLC-DADquantified rosmarinic acid, highest in ethyl acetate extract (580.93 ± 1.12 mg/g). Pearson correlation analyses demonstratedstrong positive associations between phenolic content and biological activities, supporting the pharmaceutical, cosmetic, andindustrial potential of S. hortensis.