Antioxidant, antiepileptic, and anticholinergic properties of 4′,5,7-Trihydroxy-3,6-dimethoxyflavone as natural phenolic compound: a toxicology approach

Durmaz L.

Toxin Reviews, vol.40, no.3, pp.292-299, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.1080/15569543.2019.1610894
  • Journal Name: Toxin Reviews
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Applied Science & Technology Source, BIOSIS, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, Veterinary Science Database
  • Page Numbers: pp.292-299
  • Keywords: 4′,5,7-Trihydroxy-3,6-dimethoxyflavone, acetylcholinesterase, antioxidant activity, enzyme inhibition, metabolic enzymes
  • Erzincan Binali Yildirim University Affiliated: Yes


© 2019 Informa UK Limited, trading as Taylor & Francis Group.In this work, the antioxidant properties of 4′,5,7-trihydroxy-3,6-dimethoxyflavone using several bioanalytical assays is investigated in vitro. These methods are Cu2+-Cu+ reducing, Fe3+-Fe2+reducing, DPPH• scavenging, and ABTS•+ scavenging. The IC50 values of the 4′,5,7-trihydroxy-3,6-dimethoxyflavone for DPPH• and ABTS•+ scavenging assays were 9.36 and 3.55 μg m–1, respectively. Additionally, 4′,5,7-trihydroxy-3,6-dimethoxyflavone compound recorded best inhibitory effects against human carbonic anhydrase (hCA) I and II isoenzymes, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzymes. 4′,5,7-Trihydroxy-3,6-dimethoxyflavone had K i value of 389.56 ± 29 nM against hCA I and 306.90 ± 41 nM toward hCA II, respectively. Also, the K i values of AChE and BChE were found as 1005.37 ± 117 nM and 814.22 ± 87 nM, respectively.