A Biochemical Approach for Hedysarum candidissimum from Turkey: Screening Phytochemicals, Evaluation of Biological Activites, and Molecular Docking Study

Altay A., Yeniçeri E., Taslimi P., Taşkın Tok T., Yılmaz M. A., Köksal E.

CHEMISTRY AND BIODIVERSITY, cilt.19, ss.1-18, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/cbdv.202200348
  • Dergi Adı: CHEMISTRY AND BIODIVERSITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-18
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

This studywas designedto screenthe phytochemicalcompositionand investigatethe biologicalactivitiesofHedysarumcandidissimumextractsand also supportthe resultswithmoleculardockingstudies.LC/MS/MSanalysisrevealedthe presenceof 22 phytochemicalconstituents(mainlyphenolicacids,flavonoids,andflavonoidglycosides)in the plantstructure.The methanolextractexhibitedthe strongestantioxidantactivityamongall the extractswithits strongDPPHradicalscavengingand iron reducingcapacity,as well as highphenolicand flavonoidcontents.Additionally,it was foundto be the mostpromisingacetylcholinesterase(AChE:IC50: 93.26μg/mL)andα-glycosidase(AG:IC50: 28.57μg/mL)inhibitoryactivities,supportedby the majorphenolicsof the speciesthroughin silicostudies.Ethylacetateextracthad the strongestcytotoxiceffecton HT-29 (IC50: 63.03μg/mL)and MDA-MB-453(IC50: 95.36μg/mL)cancercell lines.Both extractsexhibitedconsiderableapoptoticand anti-migrativeeffectson HT-29cells.The investigationsprovidephyto-analyticaland bio-pharmacologicalresultswhichcan be extendedbyin vivostudiesin the future.