The effects of norepinephrine on lactoperoxidase enzyme (LPO)


ŞİŞECİOĞLU M., GÜLÇİN İ., ÇANKAYA M., ATASEVER A., ÖZDEMİR H.

SCIENTIFIC RESEARCH AND ESSAYS, vol.5, no.11, pp.1351-1356, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 11
  • Publication Date: 2010
  • Journal Name: SCIENTIFIC RESEARCH AND ESSAYS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1351-1356
  • Erzincan Binali Yildirim University Affiliated: Yes

Abstract

Norepinephrine, a hormone and a neurotransmitter, was known as catecholamine. In the present study, we examined the inhibitory effect of norepinephrine on lactoperoxidase enzyme purified from bovine milk. Lactoperoxidase (LPO; E.C.1.11.1.7) was purified from bovine milk with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. LPO was purified with a yield of 31.5%, a specific activity of 30.33 EU/mg proteins and 20.77 purification fold. To determine enzyme purity, SDS-PAGE was performed and single band was observed. The R-z (A(412)/A(280)) value for LPO was 0.9. The effect of norepinephrine on lactoperoxidase was determined using ABTS as a chromogenic substrate. The half maximal inhibitory concentration (IC50) value norepinephrine was found to be 67.2 mu M. Also, inhibition constant (K-i) for norepinephrine was found to be 62.0 mu M. Norepinephrine was found as noncompetitive inhibitor.