Comparative Phytochemical Profiling and α-Glucosidase Inhibitory Potential of Acca sellowiana (O.Berg) Burret Plant Parts: Antioxidant Activity Supported by LC–MS/MS and Molecular Docking
Chemistry and Biodiversity, vol.23, no.4, 2026 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 23 Issue: 4
- Publication Date: 2026
- Doi Number: 10.1002/cbdv.202503588
- Journal Name: Chemistry and Biodiversity
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
- Keywords: Acca sellowiana, Anticholinesterase, Antidiabetic, Antioxidant, LC–MS/MS
- Erzincan Binali Yildirim University Affiliated: Yes
Abstract
Comparative data on different parts of Acca sellowiana and their enzyme-inhibitory mechanisms remain limited. This study evaluated flower, fruit, leaf, and branch extracts using bioactivity-guided LC–MS/MS and molecular docking to clarify their antioxidant and antidiabetic potential. Total phenolic, flavonoid, and tannin contents were measured, antioxidant activity was assessed by DPPH and ABTS assays, and inhibition of α-glucosidase, α-amylase, AChE, BChE was determined. The most active extract was analyzed by validated LC–MS/MS, major compounds were docked with target enzymes. Leaf extract showed the highest phenolics (89.12 µg GAE/mg), flavonoids (1010.67 µg RE/mg), and tannins (152.44 µg TAE/mg). Branch and leaf exhibited strongest antioxidants (DPPH 61.8%/55.5%; ABTS 51.1%/57.2%), surpassing α-tocopherol. Branch extract showed the most potent α-glucosidase inhibition (IC50 107.9 µg/mL vs. acarbose 3081.8 µg/mL). LC–MS/MS revealed high quercetin, epigallocatechin gallate, ellagic, and gallic acids. Results highlight organ-dependent activity and identify plant as a source of natural α-glucosidase inhibitors.