This survey was carried out on the genus Macrocheles in Kelkit Valley, Turkey, during 2007- 2012. Seven species, M. muscaedomesticae (Scopoli, 1772); M. glaber (Müller, 1860); M. perglaber Filipponi & Pegazzano, 1962; M. scutatus (Berlese, 1904); M. merdarius (Berlese, 1889); M. subbadius (Berlese, 1904); and M. robustulus (Berlese, 1904), were recorded from the valley. M. perglaber, M. scutatus, and M. subbadius are first records for the Turkish fauna. Original drawings and diagnoses for all known species of the genus from the valley are included. In addition, an identification key to the Turkish species of Macrocheles is provided here.