Akademik Veri Yönetim Sistemi
6. EBAT Eurasia Biochemical Approaches & Technologies, Tokat, Türkiye, 24 - 27 Ekim 2024, ss.103
In this study, the antioxidant and cytotoxic activities of the water and methanolic extracts of the plant Potentilla recta was investigated. Antioxidant activities were assessed on the basis of total phenolic and total flavonoid contents, DPPH radical scavenging and ferrous ion reducing antioxidant power capacities.1 The cytotoxic activities of the extracts were tested on HEP3B, A549, HeLa and HT-29 human cancer cell lines using the XTT method.2-3 The Folin Ciocalteu and aluminium chloride studies demonstrated that the methanol extract from the plant exhibited a higher concentration of phenolic and flavonoid contents. Additionally, the methanolic extract demonstrated a significantly enhanced iron ion reducing capacity, which correlated with the observed increase in phenolic content. The XTT analysis demonstrated that the water and methanolic extracts exhibited considerably enhanced cytotoxicity against diverse human cancer cell lines, with markedly reduced IC50 values. The methanol extract of the plant demonstrated a markedly potent IC50 value (IC50=78.29±0.043 µg/mL) against the HEP3B cancer cell line, whilst the water extract exhibited the most efficacious result with the lowest IC50 value against both HT-29 (IC50=37.13±0.017 µg/mL) and HeLa (IC50=21.20±0.015 µg/mL) cell lines. The results were found to be ineffective for the A549 cell line. These preliminary results suggest that the methanol extract of the plant, with its rich phytochemical content, has a potent in vitro antioxidant cytotoxic effect, opening the door to in vivo anticancer studies.