Akademik Veri Yönetim Sistemi
I. INTERNATIONAL CONGRESS on MEDICINAL and AROMATIC PLANTS: “NATURAL and HEALTHY LIFE”, Konya, Türkiye, 9 - 11 Mayıs 2017, ss.900
The aim of this study was to isolate the available secondary metabolites in Tanacetum alyssifolium plants by column chromatography and determine their structures with spectroscopic methods. It was also aimed to examine the antioxidant and anticanserogen properties of Tanacetum alyssifolium. For this purpose, Tanacetum alyssifolium plants was collected from the foothills of Munzur Mountains in Erzincan province and dried at room temperature. The upper part of ground plant was extracted with ethyl acetate/ butanol solvent system. The ethyl acetate extract was subjected to the procedure of column chromatography. The structure of the isolated compound with column chromatography method was elucidated by spectroscopic techniques (1D-NMR, 2D-NMR, HPLCTOF). The isolated compound was determined as 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,6- dimethoxy-4H-chromene-4-on (axillarin). The antioxidant capacity of the isolated compound was evaluated with CUPRAC method and DPPH radical scavenging activity tests, respectively. Trolox was used as standard antioxidant. In terms of the iron (Fe+3) reduce ability, axillarin compound was found to have more reduction potential than standard trolox and when compared the cupper (Cu+2) reduce ability, axillarin compound displayed less reduction potential. However, axillarin and trolox showed close results in terms of DPPH radical scavenging capacities. For anticanserogen tests, 3 different concentrations of axillarin compound were prepared and applied to HeLa cells in order to determine the concentration at which the maximal activity was shown. The highest anticanserogen activity was observed at 50 μg/mL and reached at 43 hours.