Research Information System
9th Joint Meeting of AFERP, ASP, GA, JSP, PSE and SIF, Copenhagen, Denmark, 24 - 27 July 2016, vol.82, (Summary Text)
Medicinal plants have been used in different countries for treatment of diabetes mellitus for many years [1]. α-Glucosidase and α-amylase are the key enzymes in digestion of carbohydrates. The medicinal plants with α-glucosidase and α-amylase inhibitory activities are useful in the treatment of diabetes [2]. Artemisia dracunculus L. (tarragon) is an aromatic plant in the Asteraceae family. It is cultivated in southern Europe, Russia, the United States and Turkey. Tarragon has been used in culinary traditions [3]. The infusion of aerial parts of the plant has been used in Turkish folk medicine as a digestive, diuretic, carminative, appetitive, emmenagogue, vermifuge and antidiaretic [4], and have shown to display antibacterial, antifungal, antiplatelet, anti-inflammatory, antihyperglycemic, antidiabetic, antioxidant, hepatoprotective, and anticonvulsant activities [3,5]. It has secondary metabolites such as coumarins, alkamides, flavonoids, chalcones and phenylpropanoids [3,6]. In our study, in vitro α-glucosidase and α-amylase inhibitory activities of a methanol extract of aerial parts from cultivated A. dracunculus fractions of different polarity from the methanol extract and the isolated compounds from these fractions were evaluated. The n-hexane and ethyl acetate fractions showed α-glucosidase inhibitory effect at IC50 values 0.90 and 0.95 mg/mL, respectively. A coumarin, herniarin isolated from the n-hexane extract showed α-glucosidase inhibition with IC50 = 2.1 mg/mL. A phenylpropanoid containing fraction from n-hexane extract showed higher α-glucosidase inhibitory activity with IC50 = 0.20 mg/mL. Standard compound acarbose displayed 26% inhibitory activity against α-glucosidase (IC50= 2.3 mg/mL). In addition, the n-butanol extract and its constituent rutin were inactive against α-glucosidase at all tested concentrations. None of the tested extracts or compounds inhibited α-amylase.
Acknowledgements: This work was supported by the The Foundation of Atatürk University (2015/324).
Keywords: Diabetes mellitus, Artemisia dracunculus, α-glucosidase, α-amylase.
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