Fluorescence interactions of a novel chalcone derivative with membrane model systems and human serum albumin

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Yıldırım B., Beşer B. M., Ulaş Çolak N., Altay A., Yaşar A.

BIOPHYSICAL CHEMISTRY, cilt.290, ss.1-12, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 290
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.bpc.2022.106879
  • Dergi Adı: BIOPHYSICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-12
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

A novel chalcone derivative (4-(5-(pyridin-2-yl)-4,5-dihydro-1H-pyrazol-3-yl) phenol (PDP) was synthesized,

characterized and investigated for its potential as a fluorescent probe. The structure of the synthesized molecule

was determined by FTIR, 1H NMR, 13C NMR and LC-MS/MS. The interactions of PDP with fluorescent dyes in

aqueous SDS environment and HSA were studied by using fluorescence resonance energy transfer (FRET)

technique and steady-state and time-resolved fluorescence spectroscopy techniques. In addition, the cytotoxic

effects of PDP against various cell lines (MCF -7, HT-29, and 3 T3-L1) as well as their corresponding healthy cell

lines were tested by MTT assay and visualization of FRET efficiency of PDP in vitro was monitored by confocal

microscopy. MTT assay showed that PDP has no significant cytotoxic effect on HT-29 cancer cells and moderate

cytotoxicity on MCF -7 and 3 T3-L1 cells even at a concentration of 250 μM. Combining confocal microscopes

with the FRET technique showed that PDP significantly stained the cytoplasm of MCF -7 cell lines. These results

suggest that PDP could be used in fluorescence microscopy for cell staining.