Akademik Veri Yönetim Sistemi
7. Internatıonal Cappadocia Scientific Research Congress, Nevşehir, Türkiye, 2 - 04 Ağustos 2025, ss.59-60, (Özet Bildiri)
In this study, comprehensive phytochemical and biological analyses were performed on three taxa of the genus Hedysarum L., which are naturally distributed in the flora of Türkiye and have been traditionally used in ethnomedicine. The species investigated were Hedysarum candidissimum Freyn, Hedysarum erythroleucum Boiss., and Hedysarum yilmazunalii Kandemir, F. Yıldız & H.İ. Turkoğlu. The principal objective of this investigation was to determine the total phenolic and flavonoid contents in the methanolic extracts obtained from the floral parts of the selected species and to investigate their antioxidant capacities. Antioxidant activity was measured using the DPPH· radical scavenging assay and the FRAP method. In addition, LC-MS/MS analyses were performed to characterize the chemical composition of the extracts. Among the species examined, H. erythroleucum showed the highest total phenolic content (106.40±1.07 mg GAE/g), total flavonoid content (58.99±1.09 mg QE/g), and FRAP value (168.67±0.79 μg TE/g), while exhibiting the lowest DPPH· IC50 (12.32±0.21 μg/mL). In order to better evaluate the antioxidant activity, the obtained results were comparatively analyzed using four standard reference antioxidants: Trolox, BHA, BHT, and ascorbic acid. LC-MS/MS analysis involved the detection and quantification of 56 standard phenolic compounds. Quinic acid was identified as the major compound in the floral extract of H. yılmazünalii, with a concentration of 236.013 mg/analyte/g extract. These findings indicate that antioxidant capacity varies significantly among species and are further supported by the LCMS/MS data. Overall, the results suggest that the examined Hedysarum species represent valuable endemic phytochemical reservoirs characterized by high antioxidant capacity and elevated levels of phenolic compounds. In addition, the analytical approach adopted for the comparative chemical profiling of species may serve as a reliable foundation for future research in pharmacology and biochemistry.