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INTERNATIONAL MULTIDISCIPLINARY SYMPOSIUM ON FRUG RESEARCH AND DEVELOPMENT, Erzurum, Turkey, 5 - 07 October 2017, pp.37, (Full Text)
Cotinus genus is represented in Turkey with one species [1]. Cotinus coggygria Scop. (Anacardiaceae), commonly known as “smoke tree”, is generally seen as small trees or large shrubs [2] and grows mainly in South and Central Europe, South Russia, Crimea, Caucasia, Latakia and Turkey [1].
In Turkish folk medicine, the decoction of C. coggygria leaves are used against Diabetes mellitus [3] which rises the risk of cardiovascular, renal and neurologic diseases [4]. Several parts like shoots, flowers, leaves and stem of C. coggygria contain biologically active constituents which are mainly polyphenols, flavonoids and tannins [2].
The current study describes the bioguided fractionation and isolation assay for C. coggygria. The ethyl acetate extract, due to its significant α-glucosidase inhibitory effect (IC50 = 0.0082 ± 0.01 mg/mL), was selected for isolation. As a result of this study five phenolic compounds: gallocatechin (1), methyl gallate (2), myricetin-3-O-α-L-rhamnoside (3), myricetin-3-O-β-D-galactoside (4) and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (5) were isolated from this active fraction. The structures of these compounds were determined by extensive spectroscopic analyses. Compound 5 showed significant α-glucosidase inhibitory activity with 0.0015 mg/mL IC50 value among the tested compounds, when compared to the reference standard compound acarbose (3.3642 ± 0.12 mg/mL). The present study has given supporting evidence to verify the ethnomedical use of C. coggygria Scop. against Diabetes mellitus.