Isolation and Characterization of Chlorogenic Acid from Inula Heterolepis and Quantification in Some Plants Growing in Erzincan, Turkey

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Akşit H., Şimşek S., Akşit Z., Köksal E.

International Eurasian Conference on BioTechnology and BioChemistry (BioTechBioChem 2020), Ankara, Türkiye, 16 - 18 Aralık 2020, ss.791-795

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.791-795
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

The chlorogenic acid (CA) was purified using preparative HPLC after fractionation of the methanol extract of Inula heterolepis over Sephadex LH-20 for the first time. The structure was elucidated using NMR spectra. The CA content of both I. heterolepis and other plants that grow in Erzincan was quantified using HPLC-UV at 330 nm. Chlorogenic acid was not detected in 11 plants including Onobrychis galegifolia, Onobrychis nitida, Ebenus macrophylla, Thymus convulutus, Chrysathesium stellerioides, Thymus pectinatus, Origanum acutidens, Rhinanthus angustifolius, Thymus vulgaris, Alchemilla erzincanensis, and Scrophularia libanotica. In addition to Inula heterolepis, Scorzonera aucherina, Helichyrsum arenarium, Helichyrsum arenarium subs. erzincanicum, Trachomitum venetum, Achillea teretifolia, Helichrysum plicatum, Tanacetum balsamita, and Vinca sonerii, chlorogenic acid content was found in various amount ranged from 9.60 to 48.92 mg/g extract.