Akademik Veri Yönetim Sistemi
Uluslararası 9. İlaç Kimyası Kongresi (9th Drug Chemistry Conference), Antalya, Türkiye, 8 - 11 Nisan 2021, ss.1-4
In the present study, total phenolic and flavonoid contents, antioxidant activity, cytotoxicity,
migration and apoptotic effects of the various extracts from the aerial parts of Consolida
orientalis were investigated. Total phenolic (TPC) and total flavonoid (TFC) content of the
extracts were determined by Folin ciocalteu and aluminium chloride colorimetric assays,
respectively. Antioxidant activity was evaluated by DPPH radical scavenging and ferric ion
reducing antioxidant power (FRAP) assays. Cytotoxicity of the extracts was investigated over
human colorectal adenocarcinoma (HT-29) and human breast adenocarcinoma (MCF-7) cells
by XTT assay. Migration and apoptotic properties were determined by wound healing and flow
cytometry analysis, respectively. Folin ciocalteu and aluminium chloride assays showed that
the methanolic extract was the richest in phenolic and flavonoid content, followed by water
and hexane extracts, respectively. In parallel to their phenolic content, methanolic extract
displayed the highest DPPH radical scavenging and ferric ion reducing capacity while the
hexane extract showed the lowest antioxidant activity. XTT assay results indicated that the
methanolic extract possessed the highest cytotoxicity against all the tested cancer cell lines
with its lower IC50 values. Flow cytometry and wound healing analysis performed with the
methanolic extract over HT-29 cells showed that this extract prevents the migration of HT-29
cells by directing them to apoptosis. In conclusion, all these results provide a significant basis
for the utilization of Consolida orientalis as an alternative to chemotherapeutic drugs used in
the treatment of breast and colon cancer with lower side effects as well as being a source for
other preclinical studies to be performed with this species.
Keywords: Consolida orientalis, Phenolic content, Antioxidant activity, Cytotoxicity