Enhancing purple rice nutritional profile, γ-amino-butyric acid and bioactive substances by germination time optimization and ultrasonic techniques


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Çebi K., Yangılar F.

CEREAL RESEARCH COMMUNICATIONS, vol.54, no.1, pp.419-433, 2026 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 54 Issue: 1
  • Publication Date: 2026
  • Journal Name: CEREAL RESEARCH COMMUNICATIONS
  • Journal Indexes: Scopus, Science Citation Index Expanded (SCI-EXPANDED), BIOSIS
  • Page Numbers: pp.419-433
  • Open Archive Collection: AVESIS Open Access Collection
  • Erzincan Binali Yildirim University Affiliated: Yes

Abstract

Purple rice (Oryza sativa L.), an antioxidant and a functional resource for humans, contains anthocyanins. In this study,

germination periods of purple rice (0, 12, 24, 36, and 48 h) were evaluated to assess changes in bioactive compounds,

antioxidant activity, and overall quality parameters. Germination was found to significantly enhance both bioactive components

and antioxidant properties. At 24 h of germination, the highest levels of total anthocyanins (477.78 mg/kg), DPPH

radical scavenging activity (57.1%), and γ-amino-butyric acid (9.64 ± 0.22 mg/100 g) were observed. At 12 h, maximum

values were recorded for flavonoid content (6.69 mg QE/g), ABTS•+ activity (76.99%), and FRAP value (43.83 mg

TE/g). The highest total phenolic content was detected at 48 h of germination. Furthermore, enzymatic saccharification

using α-amylase and α-glucoamylase led to a marked increase in sugar content, including total sugar, reducing sugar, and

glucose, in purple rice extract, with the extent of increase dependent on enzyme incubation time. These findings suggest

that germinated purple rice holds significant potential for enhancing the nutritional quality of functional food products.