Effect of thiamine pyrophosphate on cyclophosphamide-induced oxidative ovarian damage and reproductive dysfunction in female rats

Özer M., İnce S., Gündoğdu B., Aktaş M., Uzel K., Gürsul C., ...Daha Fazla

Advances In Clinical And Experimental Medicine, cilt.31, ss.1-9, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31
  • Basım Tarihi: 2022
  • Doi Numarası: 10.17219/acem/142535
  • Dergi Adı: Advances In Clinical And Experimental Medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.1-9
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Evet

Özet

Background. Cyclophosphamide is a drug used in various types of cancer. It can cause oxidative and inflammatory ovarian damage and infertility. Thiamine pyrophosphate (TPP) tobe investigated for its effect oncyclophosphamide-induced ovarian damage and reproductive dysfunction inthepresent study istheactive metabolite of thiamine. It has been shown that TPP protects organs and tissues from oxidative stress and proinflammatory cytokine damage. Objectives. To investigate the effect of TPP against the ovarian damage and reproductive dysfunction caused bycyclophosphamide inrats. Materials and methods. Albino Wistar type female rats were divided into healthy control (HG), cyclophosphamide (CYC) and TPP + cyclophosphamide (TPPC) groups (for each group, n=12). Thiamine pyrophosphate atadose of25 mg/kg was injected intraperitoneally (ip.) intheTPPC group, and 0.9% NaCI solution was injected ip. intheCYC and HG groups. One hour after theinjection, 75mg/kg ofcyclophosphamide was administered ip. intheTPPC and CYC groups. This procedure was repeated once aday for 30days. At theend of this period, 6rats from each group were euthanized with ahigh dose ofanesthetic (50 mg/kg of sodium thiopental). Biochemical and histopathological examinations were performed ontheextracted ovarian tissue. Theremaining animals were kept inthelaboratory with mature male rats for 2 months for reproduction. Results. Thiamine pyrophosphate significantly decreased thecyclophosphamide-induced increase inthelevels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1β). In addition, TPP decreased the severe histopathological damage associated with cyclophosphamide intheovarian tissue and prevented infertility. Conclusions. Our experimental results have suggested that TPP could be beneficial in the treatment of cyclophosphamide-induced ovarian injury and infertility.