5. Bilsel Internatıonal Sur Scientific Researches Congress, Diyarbakır, Türkiye, 19 - 20 Temmuz 2025, ss.258-259, (Özet Bildiri)
Phenolic compounds are considered one of the most prominent groups of natural antioxidants, constituting a diverse class of secondary metabolites widely distributed in plants. These polyphenolic structures are widely distributed in all parts of plants—including roots, stems, leaves, fruits, and seeds—and comprise major subclasses such as flavonoids, cinnamic acid derivatives, coumarins, tocopherols, and phenolic acids. It has been scientifically demonstrated that these compounds have the potential to protect oxidation-prone components in foods from oxidative degradation induced by free radicals. Given these protective properties, medicinal and aromatic plants characterized by high phenolic content are increasingly recognized for their potential use as natural additives, particularly in improving the sensory attributes of food products such as taste and aroma.
Plants of the genus Hedysarum L. possess various pharmacological properties, providing multiple applications in pharmacy and medicine. Accordingly, detailed investigation of the biological characteristics and potential uses of species within this genus is of significant scientific interest. In this study, the endemic species Hedysarum candidissimum, native to the flora of Erzincan, was collected from four distinct locations in the Kemah district: Acemoğlu, Dumanlı, Soğuksular, and Yahşiler 1 and 2. The collected plant materials were divided into root, stem, and leaf organs, and their phenolic compound contents were analyzed using the LC-MS/MS technique.
The LC-MS/MS analysis indicated that the highest phenolic compound concentrations in H. candidissimum were found in different plant organs from various sampling locations. In leaf samples, isoquercitrin was found at the highest concentration (97.454 mg/analyte/g extract) in the Yahşiler-2 locality, while stem samples from the same locality were found to have the highest quinic acid concentration (172.151 mg/analyte/g extract). In root samples, the highest quinic acid concentration (29.135 mg/analyte/g extract) was observed in the Soğuksular locality.
These findings provide valuable insights into the potential pharmacological applications of H. candidissimum. In addition, the approach of analyzing different plant organs and sampling from multiple geographic locations using LC-MS/MS provides a comprehensive methodology for characterizing the phytochemical diversity of the species, thus serving as a methodological reference for future research in the field