Akademik Veri Yönetim Sistemi
Turkish Society of Physiological Sciences, 46th Turkish Physiology Congress, İstanbul, Türkiye, 8 - 10 Ekim 2021, ss.69
AIM: High ambient temperature beyond the comfort zone or thermoneutral zone range causes environmental heat stress. A large amount of free radicals are produced as a result of the organism's response to stress. Lipid peroxidation (LPO) is one of the main events caused by oxidative stress. The purpose of this research; To analyze the effect of heat stress exposure on LPO in rat heart, duodenum, brain and testis homogenates.
METHODS: In the study, 18 male Sprague Dawley rats were used as animal material. Ethics committee approval was obtained from Atatürk University Animal Experiments Local Ethics Committee for the study. The rats were divided into 3 groups and the six experimental animals forming the groups were kept in the same cage. First group, Thermo-neutral (TN; Untreated without heat stress and 24±2°C normal ambient temperature and 60–70% relative humidity (Control)) group, second group heat stress 1 [SS1; 8 hours a day for 14 days at 30 ⸰C and relative humidity (30-35%) and the third group heat stress 2 [SS2; 8 hours a day for 14 days at 35 ⸰C and relative humidity (30-35%). At the end of the heat stress application, the rats were sacrificed under ether anesthesia. Tissues were collected sterile, homogenized and centrifuged. The supernatant was aliquoted and analyzed using the ELISA Kit.
RESULTS: When LPO values were arranged as TN, SS1 and SS2 according to tissues; for heart 12.24 ± 0.13, 12.42 ± 0.14 and 12.72 ± 0.14 ng/mL; for the duodenum 4.75 ± 0.050, 7.25 ± 0.073 and 8.40 ± 0.091 ng/mL; for the brain, 9.22 ± 0.095, 9.66 ± 0.097, and 11.72 ± 0.014 ng/mL, and for the testis 8.48 ± 0.097, 10.22 ± 0.018, and 11.33 ± 0.017 ng/mL.
CONCLUSION: Application of heat stress increases oxidative stress in heart, duodenum, brain and testis tissues by causing an increase in LPO levels.