Akademik Veri Yönetim Sistemi
7th Drug Chemistry Conference: Design, Synthesis, Production and Standardization of Drug Active Substances, Antalya, Türkiye, 14 - 17 Mart 2019, ss.24
Deinococcus radiodurans is a red pigmented, non-pathogenic bacterium that is highly resistant to
reactive oxygen species, ionizing and UV-C radiation.D. radiodurans synthesize deinoxanthin, a unique
ketocarotenoid. Deinoxanthin has a pro-apoptotic effect and can also have a pro-apoptotic effect
under certain conditions. Studies on HepG2 (human hepatoma), HT-29 (colon cancer) and PC-3
(prostate cancer) cell lines have shown that deinoxanthin induces apoptosis by increasing intracellular
reactive oxygen1
.
HER2 overexpression occurs in 20 to 30% of patientswith invasive breast carcinomas. Overall, HER2
overexpressed tumors are associated with aggressive disease,a faster rate of growth,high mortalityand
a poorer prognosis.
Trastuzumab is a monoclonal antibody-based therapeutic used in breast, gastric and gastroesophageal
cancer in which HER2 is overexpressed.However, giving trastuzumab alone in treating breast cancer
do not enough2
.
In the present study, an investigation of the synergistic effect of deinoxanthin and Trastuzumab on
breast cancer MDA-MB-453 cell. It has been studied that the gene expression level of some apoptotic
proteins (Bax, Caspase-3, APAF-1) with the human housekeeping gene β-actin used as a reference to
normalize data.Furthermore,the synergy was revealed withantiproliferative effect (XTT), levels of total
antioxidant capacity and the activity of some antioxidant enzymes in the deinoxanthin and
trastuzumab-applied cells.