Investigation of In Vitro Cytotoxic Effects of Different Extracts of Endemic Achillea sintenisii Against MCF-7, MDA-MB-453 and HT-29 Human Cancer Cell Lines


Altın S., Köksal E., Alp C., Altay A., Akşit H., Ulutaş Y.

3rd Eurasia Biochemical Approaches & Technologies Congress (EBAT), Antalya, Turkey, 4 - 07 November 2021, pp.107

  • Publication Type: Conference Paper / Summary Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.107
  • Erzincan Binali Yildirim University Affiliated: Yes

Abstract

Medicinal and aromatic plants have been used for various purposes from past to present. The interest in natural and herbal treatment is increasing rapidly, especially due to the possible side effects of synthetic drugs. Due to the bioactive properties of medicinal plant extracts and secondary metabolites isolated from these plants, their use has increased especially in cancer treatment.1 Achillea is a plant belonging to the Asteraceae family. Contains more than 100 species worldwide. There are 50 species (56 taxa) in Turkey, 26 of which are endemic.2 Achillea species is used in folk medicine in the treatment of many ailments such as stopping blood, inflammatory conditions, pain relief, diuretic, carminative, wound healing and gastrointestinal diseases. 3 In this study, the cytotoxic effects of various extracts (ethyl acetate, methanol and hexane) obtained from the aerial parts of endemic A. sintenisii were investigated. The cytotoxicity of the extracts was investigated by XTT assay on human colorectal adenocarcinoma (HT-29) and human breast adenocarcinoma (MCF-7 and MDA-MB-453) cells. XTT assay results showed that ethyl acetate extract had the highest cytotoxicity against all tested cancer cell lines with low IC50 values. Especially for MDA-MB-453 human breast adenocarcinoma cell, an IC50 value of 83 µg/ml is a very important result. IC50 values for other cell lines MCF-7 and HT-29 were 138 µg/ml and 255 µg/ml, respectively, depending on dose increase. Since this study is the first anticancer study conducted for an endemic species, A. sintenisii, it makes our study original. The results of this study, while the high antiproliferative effect of A. sintenisii on human breast and colon cancer cells is promising for further studies, it also constitutes a source for other studies on this species