Ultraperformance liquid chromatography with electrospray ionization ion trap mass spectrometry for chondroitin disaccharide analysis


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Solakyıldırım K., Zhang Z., Linhardt R. J.

Analytical Biochemistry, cilt.397, sa.1, ss.24-28, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 397 Sayı: 1
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.ab.2009.09.031
  • Dergi Adı: Analytical Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.24-28
  • Anahtar Kelimeler: Chondroitin sulfate, Dermatan sulfate, Disaccharides, Glycosaminoglycans, Ion-pairing, LC-MS, Reversed phase
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır

Özet

Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of CS, simple, sensitive, high resolution, and robust analytical methods are needed for the analysis of CS disaccharides and oligosaccharides. An ion-pairing, reversed-phase, ultraperformance liquid chromatography (IPRP-UPLC) separation, coupled to electrospray ionization mass spectrometry with an ion trap mass analyzer, was applied for the analyses of CS-derived disaccharides. UPLC separation technology uses small particle diameter, short column length, and elevated column temperature to obtain high resolution and sensitivity. Hexylamine (15 mM) was selected as the optimal ion-pairing reagent. © 2009 Elsevier Inc.