Ultraperformance liquid chromatography with electrospray ionization ion trap mass spectrometry for chondroitin disaccharide analysis

Creative Commons License

SOLAKYILDIRIM K. , Zhang Z., Linhardt R. J.

Analytical Biochemistry, vol.397, no.1, pp.24-28, 2010 (Journal Indexed in SCI Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 397 Issue: 1
  • Publication Date: 2010
  • Doi Number: 10.1016/j.ab.2009.09.031
  • Title of Journal : Analytical Biochemistry
  • Page Numbers: pp.24-28
  • Keywords: Chondroitin sulfate, Dermatan sulfate, Disaccharides, Glycosaminoglycans, Ion-pairing, LC-MS, Reversed phase


Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of CS, simple, sensitive, high resolution, and robust analytical methods are needed for the analysis of CS disaccharides and oligosaccharides. An ion-pairing, reversed-phase, ultraperformance liquid chromatography (IPRP-UPLC) separation, coupled to electrospray ionization mass spectrometry with an ion trap mass analyzer, was applied for the analyses of CS-derived disaccharides. UPLC separation technology uses small particle diameter, short column length, and elevated column temperature to obtain high resolution and sensitivity. Hexylamine (15 mM) was selected as the optimal ion-pairing reagent. © 2009 Elsevier Inc.