Effect of codon optimization and promoter choice on recombinant endo-polygalacturonase production in Pichia pastoris

KARAOĞLAN M., ERDEN KARAOĞLAN F.

ENZYME AND MICROBIAL TECHNOLOGY, vol.139, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 139
  • Publication Date: 2020
  • Doi Number: 10.1016/j.enzmictec.2020.109589
  • Journal Name: ENZYME AND MICROBIAL TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Keywords: Aspergillus niger, Codon-optimization, Pectinase, Pichia pastoris, Protein purification, ASPERGILLUS-NIGER, DINITROSALICYLIC ACID, PROTEIN-PRODUCTION, EXPRESSION, PURIFICATION, PECTINASE, ENDOPOLYGALACTURONASE, EXTRACTION, GENE
  • Erzincan Binali Yildirim University Affiliated: Yes

Abstract

Pectinase is one of the most widely used enzymes in different fields of food industry for different purposes, such as clarification of fruit juice, extraction of vegetable oil and saccharification of agricultural substrates. The aim of this study is to produce recombinant pectinase and evaluate the effect of codon optimization and promoter selection on production.