High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis

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Laremore T. N., Ly M., Solakyıldırım K., Zagorevski D. V., Linhardt R. J.

Analytical Biochemistry, cilt.401, sa.2, ss.236-241, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 401 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.ab.2010.03.004
  • Dergi Adı: Analytical Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.236-241
  • Anahtar Kelimeler: ESI-FTMS, Heparin oligosaccharides, Preparative PAGE
  • Erzincan Binali Yıldırım Üniversitesi Adresli: Hayır

Özet

Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies. © 2010 Elsevier Inc.